mouse leukemia cell line l1210  (Procell Inc)

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 2. Transfection efficiency at different MOIs transfection. L1210 cells were transfected with different lentiviruses MOIs.

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 3. CD4, CCR5, and CyclinT1 levels in transgenic cells qRT-PCR analysis. L1210 cell was transfected with different

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transgenic Assay, Quantitative RT-PCR, Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 4. CD4, CCR5, and CyclinT1 protein expression in transgenic L1210 cells. L1210 cells transfected with lentivirus containing CD4/CCR5/CyclinT1 expressed CD4, CCR5, and CyclinT1. ∗p < 0.05 and ∗∗p < 0.01.

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Expressing, Transgenic Assay, Transfection

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 5. HIV-1 RNA levels in transgenic L1210 cells. L1210 cells transfected with the lentivirus vector containing CD4/CCR5/CyclinT1

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: Transgenic Assay, Transfection, Plasmid Preparation

Journal: Discovery Medicine

Article Title: Establishment of Transgenic Mouse Leukemia Cell Lines Expressing Human CD4/CCR5/CyclinT1 Infected with HIV-1

doi: 10.24976/discov.med.202335175.12

Figure Lengend Snippet: Fig. 6. HIV-1 RNA expression level in CEMx174 cells. (A) The HIV-1 RNA level in the supernatant. (B) Cellular HIV-1 RNA expres- sion. H-M represents cells infected with the HIV-1 collected from transgenic L1210, negative control represents cells infected with the

Article Snippet: HEK293T cell line and mouse leukemia cell line L1210 were obtained from the ATCC (American Type Culture Collection) library.

Techniques: RNA Expression, Infection, Transgenic Assay, Negative Control

Journal: Cancer Drug Resistance

Article Title: Daunorubicin can eliminate iPS-derived cancer stem cells via ICAD/CAD-independent DNA fragmentation

doi: 10.20517/cdr.2019.01

Figure Lengend Snippet: Comparison of apoptotic features among L1210, miPS-LLCcm, and Hela cells. A: Mouse leukemia L1210 cells were treated with 1 μmol/L daunorubicin for the indicated periods. Protein levels of activated caspase-3 and its substrate ICAD were determined by western blotting; B: DNA fragmentation in L1210 cells was analyzed. Cells were treated with daunorubicin for 24 or 36 h; C: DNA fragmentation in miPS-LLCcm and L1210 cells treated with staurosporine. Cells were treated with various concentrations of staurosporine for 3 h (miPS-LLCcm) or 12 h (L1210), after which DNA fragmentation was observed; D: Western blot analysis of PARP-1 and ICAD levels in staurosporine-treated cells (3 h for miPS-LLCcm and 12 h for L1210); E: ICAD levels in Hela cells treated with 1 μmol/L daunorubicin for the indicated periods were analyzed by western blotting; F: DNA fragmentation in Hela cells treated with 1 μmol/L daunorubicin

Article Snippet: The mouse leukemia cell line L1210 (JCRB cell bank, Osaka, Japan) was maintained in RPMI1640 containing 10% FBS, 2 mmol/L L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin.

Techniques: Comparison, Western Blot